VEGF/PlGF Heterodimer Antibody (146213)

• Reactivity: Hu
• Applications: WB
• Clone: 146213
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VEGF/PlGF Heterodimer Antibody (146213) Summary

• Immunogen: E. coli-derived recombinant human VEGF/PlGF heterodimer
  Ala27-Arg191 (VEGF) & Ala21-Arg149 (PIGF)
  Accession # AAM03108 (VEGF) & AAD30179 (PIGF)
• Specificity: Detects human VEGF/PlGF heterodimer in direct ELISAs and Western blots. This antibody does not detect either subunit alone. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) VEGF₁₂₁, rhVEGF₁₆₅, rhVEGF₂₀₆, rhCTGF, rhLDGF, rhPlGF, rhVEGF‑B₁₈₆, rhVEGF‑C, rhVEGF‑D, rhPDGF, rmVEGF₁₁₅, rmVEGF₁₂₀, rmVEGF₁₆₄, rmPlGF‑2, rrVEGF₁₆₄, or rzfVEGF.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGF/PlGF Heterodimer Antibody (146213)

• VEGF/PlGF Heterodimer

Background

The disulfide-linked homodimeric proteins vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF) are both members of the VEGF family of growth factors. VEGF and PlGF proteins share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. E. coli-expressed monomers of PlGF and VEGF can be refolded in vitro to form PlGF/VEGF heterodimers. The purified recombinant VEGF/PlGF heterodimers and VEGF homodimers have potent mitogenic effects on endothelial cells. However, VEGF/PlGF heterodimers display approximately 50-fold less mitogenic activity than VEGF homodimers. In contrast, PlGF homodimers have little or no mitogenic effect on endothelial cells. The presence of natural VEGF/PlGF heterodimers in the conditioned media of a number of human tumor cell lines has been demonstrated using a VEGF/PlGF heterodimer specific ELISA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGF/PlGF Heterodimer Antibody (MAB297)(2)

Publications using MAB297

• Lennikov A, Mukwaya A, Fan L et al. Synergistic interactions of PlGF and VEGF contribute to blood-retinal barrier breakdown through canonical NF kapp B activation Experimental Cell Research 2020-10-01 [PMID: 33130176]
• Lennikov A, Mukwaya A, Fan L et al. Synergistic interactions of PlGF and VEGF contribute to blood-retinal barrier breakdown through canonical NF kapp B activation Experimental Cell Research 2020-10-01 [PMID: 33130176]

Bioinformatics

• Uniprot:
  • AAM03108 (VEGF) & AAD30179 (PIGF)()