Western Blot: Ub-K63 Antibody (HWA4C4) [NBP2-00199] - In vitro linked and purified polyubiquitin chains were analyzed by SDS-PAGE followed by immunoblotting with Anti-Human Ub-K63 Purified. The antibody detects ...read more
HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin. It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains.
Protein A or G purified
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This HWA4C4 antibody has been tested by immunoblot of lysine-63-linked polyubiquitin chains. This can be used at less than or equal to 2ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63). K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed. In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair. There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Ub-K63 Antibody (NBP2-00199)(1)
We have publications tested in 1 confirmed species: Human.