Ub-K63 Antibody (HWA4C4)

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Western Blot: Ub-K63 Antibody (HWA4C4) [NBP2-00199] - In vitro linked and purified polyubiquitin chains were analyzed by SDS-PAGE followed by immunoblotting with Anti-Human Ub-K63 Purified. The antibody detects ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
HWA4C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Ub-K63 Antibody (HWA4C4) Summary

Immunogen
The immunogen for this antibody is Ub-K63.
Specificity
HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin. It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot < = 2 ug/ml
Application Notes
This HWA4C4 antibody has been tested by immunoblot of lysine-63-linked polyubiquitin chains. This can be used at less than or equal to 2ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Publications
Read Publication using
NBP2-00199 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Background

Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63). K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed. In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair. There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ub-K63 Antibody (NBP2-00199)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Showing Publication 1 - 1 of 1.
Publication using NBP2-00199 Applications Species
Liu XY, Lai F, Yan XG et al. RIP1 Kinase Is an Oncogenic Driver in Melanoma Cancer Res. 2015 Feb 27 [PMID: 25724678] (WB, Human) WB Human

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FAQs for Ub-K63 Antibody (NBP2-00199). (Showing 1 - 1 of 1 FAQ).

  1. What is the buffer composition of this product?
    • The buffer formulation of NBP2-00199 (Anti-Human Ub-K63) is 1X PBS with 0.09% sodium azide, pH 7.2, and no carrier protein.

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