Recombinant Human TRPT1 His Protein

SDS-Page: Recombinant Human TRPT1 His Protein [NBP1-78826] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human TRPT1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-253 of Human TRPT1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TRPT1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 30.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human TRPT1 His Protein

• EC 2.7.1.160
• MGC11134
• tRNA 2'-phosphotransferase 1
• tRNA phosphotransferase 1
• tRNA splicing 2' phosphotransferase 1

Background

TRPT1, also known as tRNA 2'-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TRPT1