TMIGD2 Antibody (953728) [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clone: 953728
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

TMIGD2 Antibody (953728) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects human TMIGD2/CD28H in direct ELISAs. Stains human TMIGD2/CD28H transfectants but not irrelevant transfectants in flow cytometry.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TMIGD2 Antibody (953728) [Alexa Fluor™ Plus 555]

• CD28H
• IGPR1
• IGPR-1
• MGC23244
• TMIGD2
• transmembrane and immunoglobulin domain containing 2
• transmembrane and immunoglobulin domain-containing protein 2

Background

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
4. Huang, C. et al. (2003) Nature 424:219.
5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.