TMEM87A Antibody (772807) [Unconjugated]

Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TMEM87A at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Simple Western lane view shows lysate of T47D human breast cancer cell line, loaded at 0.5 mg/mL. Specific bands were detected for TMEM87A at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, CyTOF-ready
• Clone: 772807
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TMEM87A Antibody (772807) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TMEM87A
  Ser22-Asn157
  Accession # Q8NBN3
• Specificity: Detects human TMEM87A in ELISAs and Western Blots
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMEM87A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TMEM87A Antibody (772807) [Unconjugated]

• DKFZp564G2022
• FLJ11273
• MGC33727
• PSEC0094
• TMEM87A
• transmembrane protein 87A

Background

TMEM87A (Transmembrane protein 87A) is predicted to be a 6-transmembrane, 555 amino acid (aa) glyco- and phospho-protein that is expressed on the cell surface. Its expression in the mammary gland is upregulated when there is a loss of caveolin-1 expression, which may confer susceptibility to breast cancer. Within the region used as an immunogen, human TMEM87A shares 83% aa sequence identity with mouse and rat TMEM87A. A potential isoform of 181 aa diverges C-terminal to aa 169, while another of 494 aa is divergent N-terminal to aa 70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMEM87A
• Uniprot:
  • Q8NBN3()