Mouse TLR12 Stable Cell Line

Product Discontinued

Mouse TLR12 Stable Cell Line Summary

• Description: The TLR12 stable cell line can be used for TLR12 flow cytometric calibration and detection control. TLR12 expression in this cell line has been validated by Western blotting (Fig. 1) and flow cytometry (Fig. 2).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: The TLR12 stable cell line is a stably transfected cell line which expresses full-length mouse Toll-like receptor 12 (TLR12) with an N-terminal HA tag.
• Target Species: Mouse
• Specificity: TLR12
• Selection Agent: Blasticidin
• Growth Properties: Adherent Morphology: Epithelial
• RCL Type: Stable Cell Line
• Host: HEK293
• Gene: TLR12P

Applications/Dilutions

• Readout System:
  • NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) NBP2-25286
  • Intracellular Staining Kit NBP2-26248

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the stable cell line.
- Wash hands after handling the stable cell line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Mouse TLR12 Stable Cell Line

• Gm1365
• TLR12
• toll-like receptor 12

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLR11 does not respond to known TLR ligands but instead respond specifically to uropathogenic bacteria. TLR11-deficient mice are highly susceptible to infection of the kidneys by uropathogenic bacteria, indicating that TLR11 may play a protective role in preventing infection of internal organs of the urogenital system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLR12P