Mouse TLR11 Stable Cell Line

Product Discontinued

Mouse TLR11 Stable Cell Line Summary

• Description: The TLR11 stable cell line can be used for TLR11 flow cytometric calibration and detection control. TLR11 expression in this cell line has been validated by Western blotting and flow cytometry. TLR11 expression in this cell line has been validated by Western blotting (Fig. 1) and flow cytometry (Fig. 2).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: The TLR11 stable cell line is a stably transfected cell line which expresses full-length mouse Toll-like receptor 11 (TLR11) with an N-terminal HA tag.
• Target Species: Mouse
• Specificity: TLR11
• Selection Agent: Blasticidin
• Growth Properties: Adherent Morphology: Epithelial
• RCL Type: Stable Cell Line
• Host: HEK293
• Gene: TLR11

Applications/Dilutions

• Readout System:
  • NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) NBP2-25286
  • Intracellular Staining Kit NBP2-26248

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the stable cell line.
- Wash hands after handling the stable cell line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Mouse TLR11 Stable Cell Line

• Gm287
• hgnc31753
• TLR11
• Toll like receptor 11

Background

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR11 is one of three mouse TLRs that lack a human ortholog. It is activated specifically by uropathogenic bacteria, and mice lacking TLR11 showed a much greater susceptability to uropathogenic infections, indicating a potentially important role for TLR11 in preventing infections in the urogenital system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

FAQs for TLR11 Reporter Cell Line (NBP2-26286). (Showing 1 - 1 of 1 FAQ).

1. I have purchased TLR11 stable cell line from your company and cells are now in culture. In case of other cell lines, transfected with TLRs, a special medium had to be prepared for stimulation of these cells. In the available datasheet, the listed publications and also in other literature, I can't find any information if the stimulation has to be performed in normal growth medium or in a special one. Can you give any further information regarding this problem?
   • We generally use normal culture media without selectable antibiotics when we analyze our TLR-transfected stable cell lines (such as ligand stimulation, signaling inhibitor test, agonist/antagonist test, etc). However, when you maintain the cell lines, you should not omit the selectable antibiotics in the culture media.

Bioinformatics

• Gene Symbol: TLR11