Thymine Dimer Antibody (H3) - BSA Free

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Immunocytochemistry/ Immunofluorescence: Thymine Dimer Antibody (H3) [NB600-1141] - analysis of Thymine Dimer in human keratinocytes using anti-Thymine Dimer antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, All-NA, ChSpecies Glossary
Applications DB, ELISA, ICC/IF, SB
Clone
H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
2.0 mg/ml

Order Details

Thymine Dimer Antibody (H3) - BSA Free Summary

Immunogen
tetra nucleotide containing cyclobutane thymine dimer (GTTG) conjugated to chicken gamma globulin.
Specificity
Monoclonal Anti-Thymine Dimer reacts specifically with the (5'-6') cyclobutane type of homothymine or thyminecytosine heterodimers. The antibody reacts with thymine dimers in single-stranded DNA, and has a lower affinity for the dimer in short oligonucleotides (a tail of minimum 10-20 thymine residues is required for efficient labeling of oligonucleotide probes). The product enables a sensitive and non-radioactive method for labeling, detection, and quantification of DNA fragments using ELISA, competitive ELISA, immunocytochemistry (laser-scan microscopy) and Southern immunoblotting.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Southern Blot 0.5-1 ug/ml
Application Notes
Southern Blotting, use UV induced thymine dimers of lamda phage DNA fragments
Reviewed Applications
Read 1 Review rated 4
using
NB600-1141 in the following applications:

Publications
Read Publications using
NB600-1141 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
2.0 mg/ml
Purity
Protein A or G purified

Background

Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromatographic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separation by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. This method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal antibodies reacting specifically with thymine dimer facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thymine Dimer Antibody (NB600-1141)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, IHC-P.


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ICC/IF
(2)
IHC-P
(1)
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Human
(2)
Mouse
(1)
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Review for Thymine Dimer Antibody (NB600-1141) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-1141:
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IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Thymine Dimer NB600-1141
Enlarge
4
reviewed by:
Mitchell Denning
IF Human 05/12/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedCultured Human Keratinocytes
SpeciesHuman
Lot043M4836

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NB600-1141

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Recent Reviews

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Mitchell Denning
05/12/2015
Application: IF
Species: Human