Tetanus Toxin Antibody - BSA Free

Western Blot: Tetanus Toxin Antibody [NB100-63239] - 1 ug of tetanus toxin (Sigma T3194-25UG) per well.

• Reactivity: Ba
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 5.0 mg/ml

Tetanus Toxin Antibody - BSA Free Summary

• Immunogen: Tetanus Exotoxin
• Localization: Secreted
• Specificity: NB100-63239 detects the Tetanus Toxin which is produced by the soil micro-organism Clostridium tetani. C. tetaniinfection results in its systemic growth and secretion of tetanus, which causes muscle spasms by inhibiting neurotransmitter release. The antibody also reacts with the C-fragment of tetanus toxin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TETX
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1 ug/ml
  • Western Blot 1:100-1:500
• Application Notes: By WB, it detects the complete toxin (faint 150 kDa band) , the heavy chain (main 100 kDa band) and the light chain (faint 50 kDa band).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 5.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tetanus Toxin Antibody - BSA Free

• Tentoxylysin

Background

Tetanus Toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2. The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TETX