TBC1D21 Antibody (OTI2A6)

Western Blot: TBC1D21 Antibody (2A6) [NBP2-01311] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D21 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D21.

Immunohistochemistry-Paraffin: TBC1D21 Antibody (2A6) [NBP2-01311] Staining of paraffin-embedded Human pancreas tissue using anti-TBC1D21 mouse monoclonal antibody.

Flow Cytometry: TBC1D21 Antibody (2A6) [NBP2-01311] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TBC1D21 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: TBC1D21 Antibody (2A6) [NBP2-01311] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TBC1D21 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI2A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.77 mg/ml

TBC1D21 Antibody (OTI2A6) Summary

• Description: Novus Biologicals Mouse TBC1D21 Antibody (OTI2A6) (NBP2-01311) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TBC1D21(NP_699187) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TBC1D21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.77 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TBC1D21 Antibody (OTI2A6)

• MGC34741
• TBC1 domain family member 21
• TBC1 domain family, member 21

Background

TBC1D21 may act as a GTPase-activating protein for Rab family protein(s)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TBC1D21
• Entrez:
  • 161514(Human)