![Western Blot: Rhodamine Antibody (5G5) [NBP2-22472] - Analysis of various amounts of purified TAMRA-BSA protein.](http://images.novusbio.com/fullsize2/Rhodamine-Antibody-(5G5)-Western-Blot-NBP2-22472-img0001.jpg "Western Blot: Rhodamine Antibody (5G5) [NBP2-22472] - Analysis of various amounts of purified TAMRA-BSA protein.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, IP |
| Clone | 5G5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | TAMRA molecule |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation: Rhodamine Antibody (5G5) [NBP2-22472] - Analysis was performed on purified TAMRA-BSA. The antigen: antibody complex was formed by binding 5ug protein with 10ug of mouse monoclonal antibody recognizing TAMRA or Mouse IgG, whole molecule overnight on a rocking platform at 4C. The immune-complex was then captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were imaged using Typhoon 9410 Imager at 532nM, Normal Sensitivity.](http://images.novusbio.com/fullsize2/Rhodamine-Antibody-(5G5)-Immunoprecipitation-NBP2-22472-img0003.jpg "Immunoprecipitation: Rhodamine Antibody (5G5) [NBP2-22472] - Analysis was performed on purified TAMRA-BSA. The antigen: antibody complex was formed by binding 5ug protein with 10ug of mouse monoclonal antibody recognizing TAMRA or Mouse IgG, whole molecule overnight on a rocking platform at 4C. The immune-complex was then captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were imaged using Typhoon 9410 Imager at 532nM, Normal Sensitivity.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-(MG1)-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")