TAG-72 Antibody (SPM148) - IHC-Prediluted

Images

 
Immunohistochemistry-Paraffin: TAG-72 Antibody (SPM148) - IHC-Prediluted [NBP2-45174] - Formalin-paraffin colon cancer stained with TAG-72 MAb (SPM148). Note cytoplasmic and cell surface staining.

Product Details

Summary
Reactivity Hu, Rt, Bv, Ca, HaSpecies Glossary
Applications IHC, IHC-P
Clone
SPM148
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

Order Details

TAG-72 Antibody (SPM148) - IHC-Prediluted Summary

Immunogen
Membrane-enriched fraction of a human breast carcinoma liver metastasis
Localization
Cytoplasmic and cell surface
Specificity
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Alternate Names for TAG-72 Antibody (SPM148) - IHC-Prediluted

  • CA 72-4
  • Protein TAG-72
  • tag 72
  • tag-72 Protein TAG-72

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAG-72 Antibody (NBP2-45174) (0)

There are no publications for TAG-72 Antibody (NBP2-45174).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TAG-72 Antibody (NBP2-45174) (0)

There are no reviews for TAG-72 Antibody (NBP2-45174). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TAG-72 Antibody (NBP2-45174) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34741AF350
Alexa Fluor 405 NBP2-34741AF405
Alexa Fluor 488 NBP2-34741AF488
Alexa Fluor 532 NBP2-34741AF532
Alexa Fluor 594 NBP2-34741AF594
Alexa Fluor 647 NBP2-34741AF647
Alexa Fluor 700 NBP2-34741AF700
Alexa Fluor 750 NBP2-34741AF750
Allophycocyanin NBP2-34741APC
Biotin NBP2-34741B
DyLight 350 NBP2-34741UV
DyLight 405 NBP2-34741V
DyLight 488 NBP2-34741G
DyLight 550 NBP2-34741R
DyLight 594 NBP2-34741DL594
DyLight 650 NBP2-34741C
DyLight 680 NBP2-34741FR
DyLight 755 NBP2-34741IR
FITC NBP2-34741F
HRP NBP2-34741H
Janelia Fluor 549 NBP2-34741JF549
Janelia Fluor 646 NBP2-34741JF646
PE NBP2-34741PE
PerCP NBP2-34741PCP

Additional TAG-72 Products

Array NBP2-45174

Blogs on TAG-72

There are no specific blogs for TAG-72, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TAG-72 Antibody (SPM148) - IHC-Prediluted and receive a gift card or discount.