TAG-72 Antibody (SPM148) [DyLight 488]

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Product Details

Summary
Reactivity Hu, Rt, Bv, Ca, HaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM148
Clonality
Monoclonal
Host
Mouse

TAG-72 Antibody (SPM148) [DyLight 488] Summary

Immunogen
Membrane-enriched fraction of a human breast carcinoma liver metastasis
Localization
Cytoplasmic and cell surface
Specificity
Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 488
Format
DyLight 488 (A=493, E=518)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for TAG-72 Antibody (SPM148) [DyLight 488]

  • CA 72-4
  • Protein TAG-72
  • tag 72
  • tag-72 Protein TAG-72

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TAG-72 Antibody (NBP2-34741G) (0)

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Secondary Antibodies

 

Isotype Controls

Additional TAG-72 Antibody (SPM148) [DyLight 488] Products

TAG-72 NBP2-34741G

Blogs on TAG-72

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Contact Information

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