SV40 Large T Antigen Antibody (LP7N) [Janelia Fluor® 669]

• Reactivity: V-Vi
• Applications: ICC/IF, IHC
• Clone: LP7N
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 669

SV40 Large T Antigen Antibody (LP7N) [Janelia Fluor® 669] Summary

• Immunogen: Detergent insoluble fraction of SVK14 cell line (SV40 transformed neonatal keratinocytes)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Simian virus (SV40)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for SV40 Large T Antigen Antibody (LP7N) [Janelia Fluor® 669]

• Large T antigen
• Small T antigen

Background

SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.