Streptavidin Antibody [Biotin]

Western Blot: Streptavidin Antibody [Biotin] [NB120-7241] - Lane 1: Trypsin Inhibitor reduced. Lane 2: Trypsin Inhibitor non-reduced. Lane 3: Streptavidin reduced. Lane 4: Streptavidin non-reduced. Load: ~1ug per lane. Primary antibody: Primary antibody at 1:1000 for overnight at 4C. Secondary antibody: Dylight 649 conjugated Donkey anti rabbit secondary antibody at 1:10,000 for 45 min at RT. Block overnight at 4C. Predicted/Observed size: 18.8 kDa, ~15 kDa for Streptavidin, 24 kDa, ~20 kDa for Trypsin Inhibitor.

Western Blot: Streptavidin Antibody [Biotin] [NB120-7241] - Streptavidin Biotin Conjugated Antigen: Lane 1 - Biotin conjugated Streptavidin Antibody Lane 2 - Streptavidin Antibody Load: 3-fold serial dilution starting at 200 ng Secondary antibody: HRP Streptavidin at 1:40,000 for 60 min at

Product Discontinued

Streptavidin Antibody [Biotin] Summary

• Immunogen: Streptavidin [Streptomyces avidinii]
• Localization: Cytoplasmic
• Specificity: No cross reactivity occurs against avidin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:2000-1:20000
  • Western Blot 1:100-1:2000
• Application Notes: This antibody has been assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this product. Optimization of the concentration in immunoassays should be performed by the researcher.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Streptavidin [Streptomyces avidinii].

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Notes

Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Streptavidin Antibody [Biotin]

• SA V1; SA V2; Streptavidin V1; Streptavidin V2
• Streptavidin

Background

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.