Streptavidin Antibody (S8C12)

• Reactivity: NA
• Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
• Clone: S8C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Streptavidin Antibody (S8C12) Summary

• Immunogen: Streptavidin.
• Specificity: Does not cross react with egg white avidin. Binds to Streptavidin without interfering with the biotin-binding reaction
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • CyTOF-ready
• Application Notes: ELISA, Flow Cytometry, Immunohistochemistry-Paraffin/Frozen, Western Blot: Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Chemical

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Streptavidin Antibody (S8C12)

• SA V1; SA V2; Streptavidin V1; Streptavidin V2
• Streptavidin

Background

No cross-reactivity with egg white avidin. Affinity (Kd) > 1 x 108 for all MAbs. MAbs bind with high efficiency with different epitopes of streptavidin molecule. MAb S8C12 reacts with the determinant nonoverlapping with S8E4-, S10D4- and S3E11-epitopes. All MAbs bind to the streptavidin without interfering with the biotin-binding reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.