Streptavidin Antibody (S8C12)

• Reactivity: All-NA
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: S8C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Streptavidin Antibody (S8C12) Summary

• Immunogen: Streptavidin.
• Specificity: No cross-reactivity with egg white avidin. Binds with high efficiency with different epitopes of streptavidin molecule. Mab S8C12cc reacts with the determinant nonoverlapping with MAbs S8E4-, S10D4- and S3E11-epitopes. Binds to the streptavidin without interfering with the biotin-binding reaction.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • CyTOF-ready
• Application Notes: Can be used to localize streptavidin, to amplify signals in immunocytochemistry, immunohistochemistry, ELISA, flow cytometry and immunoblotting. These mouse anti-streptavidin MAbs applied to the ABC complex, previously bound to the second antibody system allow for further enhancement of signal when anti-mouse antibody conjugate is added. MAbs are effective in immunoadsorbtion procedures of streptavidin - biotinylated protein complexes. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. This antibody is CyTOF ready.

Reactivity Notes

Chemical

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Streptavidin Antibody (S8C12)

• SA V1; SA V2; Streptavidin V1; Streptavidin V2
• Streptavidin

Background

No cross-reactivity with egg white avidin. Affinity (Kd) > 1 x 108 for all MAbs. MAbs bind with high efficiency with different epitopes of streptavidin molecule. MAb S8C12 reacts with the determinant nonoverlapping with S8E4-, S10D4- and S3E11-epitopes. All MAbs bind to the streptavidin without interfering with the biotin-binding reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.