![Immunocytochemistry/Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922] - Analysis of 4% PFA fixed human embryonal carcinoma cell line Tera using anti-SSEA-5 Ab (1:1000 dilution). SSEA-5 positive cells (red) represented pluripotent population; negative cells represented differentiated population. DAPI (Blue) indicated nuclear staining.](http://images.novusbio.com/fullsize/SSEA-5-Antibody-8e11-Immunocytochemistry-Immunofluorescence-NBP2-42922-img0003.jpg "Immunocytochemistry/Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922] - Analysis of 4% PFA fixed human embryonal carcinoma cell line Tera using anti-SSEA-5 Ab (1:1000 dilution). SSEA-5 positive cells (red) represented pluripotent population; negative cells represented differentiated population. DAPI (Blue) indicated nuclear staining.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | 8e11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Mouse SSEA-5 Antibody (8e11) - Azide and BSA Free (NBP2-42922) is a monoclonal antibody validated for use in IHC, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Undifferentiated human embryonic stem cells |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: SSEA-5 Antibody (8e11) [NBP2-42922] - Pluripotent stem cell population indicated by SSEA5-staining. Human embryonic stem cells H9 were analyzed with SSEA-5 mAB (pseudo-colored in red) and control IgG (pseudo-colored in green) in flow cytometry. Each Ab was used at a 1:50 dilution in PBS.](http://images.novusbio.com/fullsize/SSEA-5-Antibody-8e11-Flow-Cytometry-NBP2-42922-img0001.jpg "Flow Cytometry: SSEA-5 Antibody (8e11) [NBP2-42922] - Pluripotent stem cell population indicated by SSEA5-staining. Human embryonic stem cells H9 were analyzed with SSEA-5 mAB (pseudo-colored in red) and control IgG (pseudo-colored in green) in flow cytometry. Each Ab was used at a 1:50 dilution in PBS.")
![Immunocytochemistry/Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922] - Analysis of 4% FA fixed human embryonic stem cells H9 using anti-SSEA-5 Ab (1:50 dilution). (A) Brightfield image (B) Dapi (C) anti-SSEA-5 Ab (D) Merged image of anti-SSEA-5 Ab and DAPI](http://images.novusbio.com/fullsize/SSEA-5-Antibody-8e11-Immunocytochemistry-Immunofluorescence-NBP2-42922-img0002.jpg "Immunocytochemistry/Immunofluorescence: SSEA-5 Antibody (8e11) [NBP2-42922] - Analysis of 4% FA fixed human embryonic stem cells H9 using anti-SSEA-5 Ab (1:50 dilution). (A) Brightfield image (B) Dapi (C) anti-SSEA-5 Ab (D) Merged image of anti-SSEA-5 Ab and DAPI")
![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](https://images.novusbio.com/images/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")