SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free

Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by NBP3-52346 (the BSA Free version of this antibody) (visualized in green). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the neuronal cell body and axon initial segment (AIS) in the cerebral cortex Magnification 100X.

Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by NBP3-52346 (the BSA Free version of this antibody) (visualized in green). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the neuronal cell body and axon initial segment (AIS) in the cerebral cortex Magnification 400X.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: N393/76
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free Summary

• Description: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N393/76 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N393/76 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human SPTBN4 (Uniprot#: Q9H254) produced recombinantly in E. Coli
• Specificity: Does not cross-react with other Beta-spectrins
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: SPTBN4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for SPTBN4 Antibody (N393/76) - Chimeric - Azide and BSA Free

• beta-IV spectrin
• KIAA1642
• QV
• spectrin beta chain, brain 3
• spectrin, beta, non-erythrocytic 4
• spectrin, non-erythroid beta chain 3
• SPNB4
• SPTBN3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPTBN4