Sleeping Beauty Transposase Antibody

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Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Sleeping Beauty Transposase Antibody Summary

Immunogen
E. coli-derived recombinant Sleeping Beauty Transposase
Specificity
Detects Sleeping Beauty Transposase in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF2798 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sleeping Beauty Transposase Antibody

  • SB Transposase

Background

SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired‑like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1).
  1. Ivics, Z. et al. (1997) Cell 91:501.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sleeping Beauty Transposase Antibody (AF2798)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, Western Blot.


Filter By Application
IHC-P
(3)
Western Blot
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF2798 Applications Species
Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition Nucleic Acids Res, 2016;0(0):. 2016 [PMID: 27913727] (Western Blot, Human) Western Blot Human
Transposon mutagenesis identifies genes that cooperate with mutant Pten in breast cancer progression Proc. Natl. Acad. Sci. U.S.A., 2016;0(0):. 2016 [PMID: 27849608] (IHC-P, Mouse) IHC-P Mouse
F Voigt, L Wiedemann, C Zuliani, I Querques, A Sebe, L Mátés, Z Izsvák, Z Ivics, O Barabas Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering Nat Commun, 2016;7(0):11126. 2016 [PMID: 27025571] (Western Blot, Human) Western Blot Human
Mann M, Black M, Jones D, Ward J, Yew C, Newberg J, Dupuy A, Rust A, Bosenberg M, McMahon M, Print C, Copeland N, Jenkins N Transposon mutagenesis identifies genetic drivers of Braf(V600E) melanoma. Nat Genet, 2015;47(5):486-95. 2015 [PMID: 25848750] (IHC-P, Mouse) IHC-P Mouse
Quintana R, Dupuy A, Bravo A, Casanova M, Alameda J, Page A, Sanchez-Viera M, Ramirez A, Navarro M A transposon-based analysis of gene mutations related to skin cancer development. J Invest Dermatol, 2013;133(1):239-48. 2013 [PMID: 22832494] (Western Blot, Mouse) Western Blot Mouse
Collier LS, Adams DJ, Hackett CS, Bendzick LE, Akagi K, Davies MN, Diers MD, Rodriguez FJ, Bender AM, Tieu C, Matise I, Dupuy AJ, Copeland NG, Jenkins NA, Hodgson JG, Weiss WA, Jenkins RB, Largaespada DA Whole-body sleeping beauty mutagenesis can cause penetrant leukemia/lymphoma and rare high-grade glioma without associated embryonic lethality. Cancer Res., 2009;69(21):8429-37. 2009 [PMID: 19843846] (IHC-P, Mouse) IHC-P Mouse

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2798

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