Sleeping Beauty Transposase Antibody (324622) [Unconjugated]

Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of intestine from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of liver from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # MAB2798) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown). Inset panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: All-Multi
• Applications: WB, IHC
• Clone: 324622
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Sleeping Beauty Transposase Antibody (324622) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant Sleeping Beauty Transposase
• Specificity: Detects Sleeping Beauty (SB) Transposase in Western blots. In Immunohistochemistry tests, this antibody stains SB transposase transgenic mouse tissue sections but not wild type mouse tissue sections.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sleeping Beauty Transposase Antibody (324622) [Unconjugated]

• SB Transposase

Background

SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired-like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1).

1. Ivics, Z. et al. (1997) Cell 91:501.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sleeping Beauty Transposase Antibody (MAB2798)(6)

Publications using MAB2798

• A Aiderus, JY Newberg, L Guzman-Roj, AM Contreras-, AL Meshey, DJ Jones, F Amaya-Manz, R Rangel, JM Ward, SC Lee, KH Ban, K Rogers, SM Rogers, L Selvanesan, LA McNoe, NG Copeland, NA Jenkins, KY Tsai, MA Black, KM Mann, MB Mann Transposon mutagenesis identifies cooperating genetic drivers during keratinocyte transformation and cutaneous squamous cell carcinoma progression PloS Genetics, 2021-08-16;17(8):e1009094. 2021-08-16 [PMID: 34398873] (IHC, Mouse)
• L Kesselring, C Miskey, C Zuliani, I Querques, V Kapitonov, A Laukó, A Fehér, A Palazzo, T Diem, J Lustig, A Sebe, Y Wang, A Dinnyés, Z Izsvák, O Barabas, Z Ivics A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming Nucleic Acids Res., 2020-01-10;0(0):. 2020-01-10 [PMID: 31777924] (Western Blot, Human)
• C Su rez-Cab, RM Quintana, A Bravo, ML Casanova, A Page, JP Alameda, JM Paramio, A Maroto, J Salamanca, AJ Dupuy, A Ram¡rez, M Navarro A transposon-based analysis reveals RASA1 is involved in triple negative breast cancer Cancer Res, 2017-01-20;0(0):. 2017-01-20 [PMID: 28108518] (IHC, Mouse)
• Takahiro Kodama Transposon mutagenesis identifies genes and cellular processes driving epithelial-mesenchymal transition in hepatocellular carcinoma Proc Natl Acad Sci USA, 2016-05-31;113(24):E3384-93. 2016-05-31 [PMID: 27247392] (IHC-P, Mouse)
• Donald A Yergeau, Clair M Kelley, Emin Kuliyev, Haiqing Zhu, Michelle R Johnson Hamlet, Amy K Sater et al. Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis Mobile DNA 12/1/2011 [PMID: 22115366]
• Vink CA, Gaspar HB, Gabriel R, Schmidt M, McIvor RS, Thrasher AJ, Qasim W Sleeping beauty transposition from nonintegrating lentivirus. Mol. Ther., 2009-05-05;17(7):1197-204. 2009-05-05 [PMID: 19417741] (Western Blot, Human)