Sleeping Beauty Transposase Antibody (324622) [Unconjugated]

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Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of liver from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # ...read more
Sleeping Beauty Transposase (SBT) was detected in perfusion fixed paraffin-embedded sections of intestine from SBT transgenic mice using 5 µg/mL Mouse Anti-Sleeping Beauty Transposase Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, IHC
Clone
324622
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Sleeping Beauty Transposase Antibody (324622) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant Sleeping Beauty Transposase
Specificity
Detects Sleeping Beauty (SB) Transposase in Western blots. In Immunohistochemistry tests, this antibody stains SB transposase transgenic mouse tissue sections but not wild type mouse tissue sections.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB2798 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sleeping Beauty Transposase Antibody (324622) [Unconjugated]

  • SB Transposase

Background

SB transposase is the catalytic component of a DNA transposition system that catalyzes the excision of the transposon from its original location and promotes its reintegration elsewhere in the genome. An active transposase that acts on transposon sequences found in vertebrate genomes was reconstructed from consensus sequences of several mutated and inactive Tc1/mariner family transposase genes in salmonid fish. The entire 340 amino aa transposase sequence contains a paired-like domain with a leucine zipper for DNA binding, a nuclear localization sequence (NLS), a glycine-rich box and a DD(34)E box. The recognition domain for transposon DNA is contained within the first 123 aa of the transposase (1).
  1. Ivics, Z. et al. (1997) Cell 91:501.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sleeping Beauty Transposase Antibody (MAB2798)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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