SIRP delta Antibody (2588B) [Unconjugated]

Western blot shows lysates of human testis tissue, mouse testis tissue, and rat testis tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat SIRP delta Monoclonal Antibody (Catalog # MAB101381) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody HAF008. A specific band was detected for SIRP delta at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using our Western Blot Buffer Group 1.

SIRP delta was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line (left panel; positive staining) and MCF‑7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human/Mouse/Rat SIRP delta Monoclonal Antibody (Catalog # MAB101381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

SIRP delta was detected in immersion fixed U937 human histiocytic lymphoma cell line (left panel; positive staining) and MCF‑7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human/Mouse/Rat SIRP delta Monoclonal Antibody (Catalog # MAB101381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol forFluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 2588B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SIRP delta Antibody (2588B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell HEK293-derived human SIRP delta
  Phe30-Arg197
  Accession # Q9H106
• Specificity: Detects human SIRP delta in direct ELISAs. Detects human, mouse, and rat SIRP delta in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRP delta Antibody (2588B) [Unconjugated]

• dJ576H24.4
• Protein tyrosine phosphatase non-receptor type substrate 1-like 2
• protein tyrosine phosphatase, non-receptor type substrate 1-like 2
• PTPNS1L2
• signal-regulatory protein delta
• SIRP delta
• SIRPD
• SIRP-Delta

Background

SIRP delta (SIRPD; Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

1. Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
2. Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
3. Huttlin, E.L. et al. (2017) Nature 545:505.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9H106()