SIRP delta Antibody (2588A) [Unconjugated]

HEK293 cells transfected with Human SIRP delta (filled histogam) or irrelevant protein (open histogram) were stained with Rabbit Anti-Human SIRP delta Monoclonal Antibody (Catalog # MAB10138) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

SIRP delta was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel; positive staining) and MCF-7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human SIRP delta Monoclonal Antibody (Catalog # MAB10138) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 2588A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SIRP delta Antibody (2588A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell, HEK293-derived human SIRP delta
  Phe30-Arg197
  Accession # Q9H106
• Specificity: Detects human SIRP delta in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SIRP delta Antibody (2588A) [Unconjugated]

• dJ576H24.4
• Protein tyrosine phosphatase non-receptor type substrate 1-like 2
• protein tyrosine phosphatase, non-receptor type substrate 1-like 2
• PTPNS1L2
• signal-regulatory protein delta
• SIRP delta
• SIRPD
• SIRP-Delta

Background

SIRPD (Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

1. Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
2. Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
3. Huttlin, E.L. et al. (2017) Nature 545:505.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9H106()