Shigatoxin 1 Antibody (13C4) - Azide Free

• Reactivity: Ba
• Applications: WB, ELISA, Func
• Clone: 13C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

Shigatoxin 1 Antibody (13C4) - Azide Free Summary

• Description: Novus Biologicals Mouse Shigatoxin 1 Antibody (13C4) - Azide Free (NBP3-11250) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The monoclonal antibody 13C4 recognizes the 1B subunit of Shigatoxin 1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: stx1B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Functional
  • Immunoassay
  • Western Blot
• Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of toxin to be inactivated.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Shigatoxin 1 Antibody (13C4) - Azide Free

• Shiga toxin 1 subunit B
• Shiga-like toxin 1
• Stx1
• STX1B
• Verotoxin 1
• VT-1

Background

The monoclonal antibody 13C4 recognizes the 1B subunit of Shiga-like toxin 1. Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share;99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: stx1B