SARS Spike Protein Antibody (7G12)

WB analysis of 293T cells transfected with expression plasmid for wild-type S and mutated forms of S (S(N1056K), S(D1128A), S(N1056K, D1128A)) using NBP3-48645 SARS-CoV S delta 3 antibody [7G12].

SARS-CoV Sdelta3 antibody detects SARS-CoV Sdelta3 protein by western blot analysis. Non-transfected (-) and SARS-CoV Sdelta3-transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV Sdelta3 antibody (NBP3-48645) diluted at 1:1000.

• Reactivity: V
• Applications: WB, IP
• Clone: 7G12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SARS Spike Protein Antibody (7G12) Summary

• Description: Novus Biologicals Mouse SARS Spike Protein Antibody (7G12) (NBP3-48645) is a monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen used to generate this antibody corresponds to SARS-CoV S3 (within S1 domain) protein (168-461 a.a.).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for SARS Spike Protein Antibody (7G12)

• E2 glycoprotein
• E2
• Human coronavirus spike glycoprotein
• Peplomer protein
• S glycoprotein
• S
• SARS coronavirus S protein
• SARS coronavirus Spike Protein
• SARS CoV S glycoprotein
• SARS CoV Spike protein
• SARS CoV
• SARS-CoV S protein
• SARS-COV Spike
• SARSCoV
• Severe acute respiratory syndrome spike glycoprotein
• Severe acute respiratory syndrome virus spike glycoprotein
• Spike glycoprotein
• VGL2

Background

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients..

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SARS Spike Protein Antibody (NBP3-48645). (Showing 1 - 2 of 2 FAQ).

1. Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
   • NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
2. I am looking for a primary antibody to sars spike protein to the new COVID-19.
   • NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.