SARS Spike Protein Antibody (1A9)

BHK-21 cells transfected with full-length SARS-CoV-2 spike were fixed in 4% paraformaldehyde at RT for 30 min.Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] diluted at 1:2000.

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco-2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] diluted at 1:1000.

SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis of infected human placenta.

Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag were used to detect the bound primary antibodies.

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS Spike Protein antibody [1A9] (NBP3-48646) (1 ug/mL). Mouse IgG antibody (HRP) was used to detect bound primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS Spike Protein antibody [1A9] (NBP3-48646) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below. Capture: SARS Spike Protein antibody [1A9] (NBP3-48646) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (1 ug/mL).

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below. Capture: SARS Spike Protein antibody [1A9] (NBP3-48646) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (1 ug/mL).

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts using SARS Spike Protein antibody [1A9] (NBP3-48646) as capture antibody at concentration of 5 ug/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] was diluted at 1:10000 and used to detect the primary antibody.

SARS Spike Protein antibody [1A9] (NBP3-48646) detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.The IHC-P was performed by HISTOWIZ.

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active)

SARS Spike Protein antibody [1A9] detects SARS-CoV-2 by immunofluorescent analysis.Sample: Vero E6 cells were infected with SARS-CoV-2 (MOI of 1) and fixed with chilled methanol/acetone.Green: SARS-CoV-2 infected Vero E6 cells stained by SARS Spike Protein antibody [1A9] (NBP3-48646).Blue: Nuclei were counterstained with DAPI.*From Zheng Z, et al. bioRxiv(2020). Shown under license agreement

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below. Capture: SARS Spike Protein antibody [1A9] (NBP3-48646) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (1 ug/mL).

Indirect ELISA analysis was performed by coating plate with 50 uL of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein at concentrations ranging from 0.0625 ug/mL to 4 ug/mL. The coated protein is detected with SARS Spike Protein antibody [1A9] (NBP3-48646) at 1 ug/mL. Mouse IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.

SARS Spike Protein antibody [1A9] (NBP3-48646) detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).The IHC-P was performed by HISTOWIZ.

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag was diluted at 1:10000 and used to detect the primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS Spike Protein antibody [1A9] (NBP3-48646) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag as detection antibody.

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag was used to detect bound primary antibody.

FACS analysis of 293T cells infected with recombinant vaccinia virus carrying the Spike gene using NBP3-48646 SARS Spike Protein antibody [1A9].

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS Spike Protein antibody [1A9] (NBP3-48646) as capture antibody at concentration of 5 ug/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts using NBP3-48646 as capture antibody at concentration of 5 ug/mL and GTX135356 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active)

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below. Capture: SARS Spike Protein antibody [1A9] (NBP3-48646) (5 ug/mL) Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (1 ug/mL).

• Reactivity: V, V
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, MS, B/N
• Clone: 1A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SARS Spike Protein Antibody (1A9) Summary

• Description: Novus Biologicals Mouse SARS Spike Protein Antibody (1A9) (NBP3-48646) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen used to generate this antibody corresponds to SARS-CoV S10 (within S2 domain) protein (1029-1192 a.a.). (SARS-CoV strain: Sin2774) The exact sequence is proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize Assay dependent
  • Electron Microscopy Assay dependent
  • ELISA Assay dependent
  • Flow Cytometry Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation Assay dependent
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for SARS Spike Protein Antibody (1A9)

• E2 glycoprotein
• E2
• Human coronavirus spike glycoprotein
• Peplomer protein
• S glycoprotein
• S
• SARS coronavirus S protein
• SARS coronavirus Spike Protein
• SARS CoV S glycoprotein
• SARS CoV Spike protein
• SARS CoV
• SARS-CoV S protein
• SARS-COV Spike
• SARSCoV
• Severe acute respiratory syndrome spike glycoprotein
• Severe acute respiratory syndrome virus spike glycoprotein
• Spike glycoprotein
• VGL2

Background

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients..

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SARS Spike Protein Antibody (NBP3-48646). (Showing 1 - 2 of 2 FAQ).

1. Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
   • NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
2. I am looking for a primary antibody to sars spike protein to the new COVID-19.
   • NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.