![Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - Non-infected (-) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:1000.](http://images.novusbio.com/fullsize/SARS-CoV-NSP8-Antibody-5A10-Western-Blot-NBP3-13528-img0002.jpg "Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - Non-infected (-) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:1000.")
| Reactivity | V, VSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | 5A10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | The immunogen used to generate this antibody corresponds to SARS virus nsp8. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SARS-CoV-NSP8-Antibody-5A10-Immunocytochemistry-Immunofluorescence-NBP3-13528-img0003.jpg "Immunocytochemistry/Immunofluorescence: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:1000. Blue: Fluoroshield with DAPI.")
![Flow Cytometry: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis. Sample: Vero E6 cells infected with SARS-CoV-2. Black: Uninfected Vero E6 cells was used as a control. Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) dilution: 1:100.](http://images.novusbio.com/fullsize/SARS-CoV-NSP8-Antibody-5A10-Flow-Cytometry-NBP3-13528-img0004.jpg "Flow Cytometry: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis. Sample: Vero E6 cells infected with SARS-CoV-2. Black: Uninfected Vero E6 cells was used as a control. Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) dilution: 1:100.")
![Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:5000.](http://images.novusbio.com/fullsize/SARS-CoV-NSP8-Antibody-5A10-Western-Blot-NBP3-13528-img0001.jpg "Western Blot: SARS-CoV NSP8 Antibody (5A10) [NBP3-13528] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (NBP3-13528) diluted at 1:5000.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")