SARS-CoV 3CL Protease Antibody

Western Blot: SARS-CoV 3CL Protease Antibody [NBP1-78110] - Shows detection of a 34-kDa band corresponding to the protein. Approx. 100 ug of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:10,000.

Product Discontinued

SARS-CoV 3CL Protease Antibody Summary

• Immunogen: The antibody was developed by immunizing rabbits with a full length recombinant protein corresponding to 3CL Protease for the Human SARS coronavirus (Genbank accession no. NP_828849.2)
• Specificity: This protein A purified antibody is directed against SARS Coronavirus 3CL Protease. A BLAST analysis was used to suggest reactivity with related Coronavirus proteins.
• Predicted Species: SARS-CoV-2 (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:50000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:2000-1:10000
• Application Notes: This protein A purified antibody has been tested for use in ELISA and by western blot. Expect a band approximately 34 kDa in size corresponding - SARS 3CL Protease by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (96%)

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water.

Alternate Names for SARS-CoV 3CL Protease Antibody

• SARS 3C-like proteinase
• SARS nsp5-pp1a/pp1ab
• SARS orf1ab polyprotein (pp1ab)
• SARS Replicase polyprotein 1a

Background

Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.