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![Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Western Blot Validation with SARS-CoV-2 3CL Protease Protein. Loading: 30 ng per lane of SARS-CoV-2 3CL Protease recombinant protein. Antibodies: SARS-CoV-2 3CL Protease, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml](http://images.novusbio.com/fullsize/SARS-CoV-2-3CL-Protease-Antibody-Western-Blot-NBP3-07062-img0001.jpg "Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Western Blot Validation with SARS-CoV-2 3CL Protease Protein. Loading: 30 ng per lane of SARS-CoV-2 3CL Protease recombinant protein. Antibodies: SARS-CoV-2 3CL Protease, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml")
![Immunohistochemistry-Paraffin: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 3CL protease antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 3L-PRO (NSP5) protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-3CL-Protease-Antibody-Immunohistochemistry-Paraffin-NBP3-07062-img0002.jpg)
![ELISA: SARS-CoV-2 3CL Protease Antibody [NBP3-07062] - Antibodies: SARS-CoV-2 3CL Protease antibody. A direct ELISA was performed using SARS-CoV-2 3CL Protease recombinant protein as coating antigen and SARS-CoV-2 3CL Protease antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-3CL-Protease-Antibody-ELISA-NBP3-07062-img0003.jpg)
![Western Blot: SARS-CoV-2 3CL Protease Antibody (HL1367) - Azide and BSA Free [NBP3-25645] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] (NBP3-25645) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25645_rabbit-sars-cov-2-3cl-protease-mab-hl1367-azide-bsa-free-2120249432843.jpg "Western Blot: SARS-CoV-2 3CL Protease Antibody (HL1367) - Azide and BSA Free [NBP3-25645] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] (NBP3-25645) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 3CL Protease Antibody (HL1367) - Azide and BSA Free [NBP3-25645] - SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] detects SARS-CoV-2 (COVID-19) 3CLpro (nsp5) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) 3CLpro (nsp5) stained by SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody [HL1367] (NBP3-25645) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-25645_rabbit-sars-cov-2-3cl-protease-mab-hl1367-azide-bsa-free-2120249422648.jpg)
![Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-07061] - Western Blot Validation with SARS-CoV-2 3CL Protease Protein. Loading: 30 ng per lane of SARS-CoV-2 3CL Protease recombinant protein (10-400). Antibodies: SARS-CoV-2 3CL Protease (NBP3-07061), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml](http://images.novusbio.com/fullsize/SARS-CoV-2-3CL-Protease-Antibody-Western-Blot-NBP3-07061-img0001.jpg "Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-07061] - Western Blot Validation with SARS-CoV-2 3CL Protease Protein. Loading: 30 ng per lane of SARS-CoV-2 3CL Protease recombinant protein (10-400). Antibodies: SARS-CoV-2 3CL Protease (NBP3-07061), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml")
![ELISA: SARS-CoV-2 3CL Protease Antibody [NBP3-07061] - ELISA Validation with SARS-CoV-2 3CL Protease Protein . Antibodies: SARS-CoV-2 3CL Protease Antibody, NBP3-07061. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein as coating antigen and the anti-SARS-CoV-2 3CL Protease antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-3CL-Protease-Antibody-ELISA-NBP3-07061-img0002.jpg)
![Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-13458] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody (NBP3-13458) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SARS-CoV-2-3CL-Protease-Antibody-Western-Blot-NBP3-13458-img0001.jpg "Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-13458] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody (NBP3-13458) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-13468] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody (NBP3-13468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SARS-CoV-2-3CL-Protease-Antibody-Western-Blot-NBP3-13468-img0001.jpg "Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-13468] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody (NBP3-13468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
