SARS-CoV-2 Variant Inhibitor Screening Kit

Serological samples were collected from two donors over a time course consisting Pre-Vaccination (Pre-Vac), or after receiving the 1st Dose or 2nd Dose of the Moderna Vaccine. Donor 1 (A-C) and Donor 2 (D-F) had different blocking antibody levels after the first and second doses. In addition, increasing amounts of blocking antibodies were present at higher samples Dilution Factors after each dose. The differences in blocking efficiency of ACE-2 association between the WT, Alpha, Beta, Gamma, or Delta variant Spike RBD was measured.

Plasma samples were collected from two donors over a time course consisting of Pre-Vaccination (Pre-Vac), or after receiving the 1st Dose or 2nd Dose of the Moderna Vaccine. Donor 1 (A-C) and Donor 2 (D-F) had different blocking antibody levels after the first and second doses. In addition, increasing amounts of blocking antibodies were present at higher samples Dilution Factors after each dose. The differences in blocking efficiency of ACE-2 association between the WT, Delta, or Omicron variant Spike RBD was measured.

• Reactivity: V
• Conjugate: Biotin

SARS-CoV-2 Variant Inhibitor Screening Kit Summary

• Additional Information: COVID-19
  Now with Omicron and Delta Variant!
• Source: N/A
• Assay Type: ELISA Type Binding Assay
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SARS-CoV-2 Variant Inhibitor Screening Kit

• Spike RBD

Background

The coronavirus Spike protein receptor binding domain (RBD) resides within the S1 subunit and is responsible for binding to host cell receptors and initiating viral infection. Past coronaviruses SARS and MERS along with the global pandemic caused by SARS-CoV-2 have sparked great interest and scientific discovery leading to new vaccines and drug development. At the heart of coronavirus biology lies the Spike protein and its receptor binding domain. The Spike RBD is a 26 kDa domain consisting of a twisted five-stranded antiparallel beta sheet and sits at the apex of each Spike protein monomer. The Spike RBD is flexible thanks to a hinge region that allows for conformational changes that expose (up or open conformation) or hide (down or closed conformation) its receptor contacts. For SARS-CoV-2, the Spike RBD recognizes and tightly binds to the human receptor ACE-2. In the closely related MERS coronavirus, human DPPIV/CD26 acts as the receptor.

FAQs for Spike RBD (VANC00B). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.