10500-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA. " class="big_lightbox"> 10500-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA. " alt="Recombinant SARS-CoV-2 Spike RBD His-tag Protein (HEK293 Expressed) (10500-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA. " class="big_thumb" />

10500-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 28-38 kDa." class="big_lightbox"> 10500-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 28-38 kDa." class="big_thumb" />

933-ZN) was measured at a concentration range between 0.37 nM and 93.5 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=2.149 nM." class="big_lightbox"> 933-ZN) was measured at a concentration range between 0.37 nM and 93.5 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=2.149 nM." class="big_thumb" />

10500-CV) binds recombinant human ACE-2 (10544-ZN) in a functional ELISA. The binding activity is approximately 3-fold greater than a top competitor’s Spike RBD protein." class="big_lightbox"> 10500-CV) binds recombinant human ACE-2 (10544-ZN) in a functional ELISA. The binding activity is approximately 3-fold greater than a top competitor’s Spike RBD protein." class="big_thumb" />

10500-CV) or a competitor’s Spike RBD protein were run under reducing or non-reducing conditions and visualized by silver staining. The R&D Systems Spike protein runs as a sharper, more definitive band than the competitor’s Spike RBD protein." class="big_lightbox"> 10500-CV) or a competitor’s Spike RBD protein were run under reducing or non-reducing conditions and visualized by silver staining. The R&D Systems Spike protein runs as a sharper, more definitive band than the competitor’s Spike RBD protein." class="big_thumb" />

HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant SARS-CoV-2 Spike RBD protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB105401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant SARS-CoV-2 Spike RBD protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB10540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for Spike RBD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Spike RBD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant HCoV-229E-S RBD. PVDF membrane was probed with 1 µg/mL of Mouse Anti-HCoV-HKU1 Spike RBD Monoclonal Antibody (Catalog # MAB10938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Spike RBD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

933-ZN) in a functional ELISA." class="big_lightbox"> 933-ZN) in a functional ELISA." alt="Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein (Catalog # 10499-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA." class="big_thumb" />

933-ZN) was measured at a concentration range between 0.73 nM and 46.7 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of K_d=7.037 nM. " class="big_lightbox"> 933-ZN) was measured at a concentration range between 0.73 nM and 46.7 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of K_d=7.037 nM. " class="big_thumb" />

HAF018). A specific band was detected for Spike RBD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Spike RBD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant HCoV-NL63 S1 RBD, recombinant SARS-CoV-2 Spike S1 RBD, recombinant HCoV-HKU1 S1 RBD, recombinant HCoV-229E S1 RBD, and recombinant HCoV-OC43 S. PVDF membrane was probed with 2 µg/mL of Mouse Anti-HCoV-NL63 Spike RBD Monoclonal Antibody (Catalog # MAB11037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Spike RBD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

10605-CV) to immobilized Recombinant Human ACE-2 Fc Chimera Protein (10544-ZN) coated at 1 μg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding." class="big_lightbox"> 10605-CV) to immobilized Recombinant Human ACE-2 Fc Chimera Protein (10544-ZN) coated at 1 μg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

10523-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA." class="big_lightbox"> 10523-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA." class="big_thumb" />

10523-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 26-32 kDa." class="big_lightbox"> 10523-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 26-32 kDa." class="big_thumb" />

933-ZN) was measured at a concentration range between 0.37 nM and 93.5 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.910 nM." class="big_lightbox"> 933-ZN) was measured at a concentration range between 0.37 nM and 93.5 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.910 nM." class="big_thumb" />

10500-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is blocked by 50 µg/mL of Mouse Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> 10500-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is blocked by 50 µg/mL of Mouse Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="In a functional flow cytometry test, Recombinant SARS-Cov-2 Spike RBD His-tagged protein (10500-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is blocked by 50 µg/mL of Mouse Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF018). A specific band was detected for Spike RBD at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Spike RBD at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant SARS-CoV-2 Spike S1 RBD. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB10827) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Spike RBD at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

10522-CV), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB10827) or (B) Mouse IgG2A Isotype Control Antibody (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> 10522-CV), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB10827) or (B) Mouse IgG2A Isotype Control Antibody (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

933-ZN) in a functional ELISA." class="big_lightbox"> 933-ZN) in a functional ELISA." alt="Recombinant SARS-CoV-2 B.1.1.7 N501Y Spike RBD His-tag (Catalog # 10730-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA." class="big_thumb" />

933-ZN) was measured at a concentration range between 0.18 nM and 47.2 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.3845 nM." class="big_lightbox"> 933-ZN) was measured at a concentration range between 0.18 nM and 47.2 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.3845 nM." class="big_thumb" />

10534-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA." class="big_lightbox"> 10534-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA." alt="Recombinant SARS-CoV-2 Spike RBD His-tag (CHO Expressed) (10534-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA." class="big_thumb" />

10534-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-40 kDa and 28-35 kDa, respectively." class="big_lightbox"> 10534-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-40 kDa and 28-35 kDa, respectively." class="big_thumb" />

933-ZN) was measured at a concentration range between 0.37 nM and 93.5 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.722 nM." class="big_lightbox"> 933-ZN) was measured at a concentration range between 0.37 nM and 93.5 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.722 nM." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Spike RBD was detected in immersion fixed Transfected & absent in Wild Type HEK293 Human Embryonic Kidney Cell Line using Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB11292) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

933-ZN) to Recombinant SARS-CoV-2 B.1.525 Spike immobilized at 0.5 ug/mL (100 µL/well)." class="big_lightbox"> 933-ZN) to Recombinant SARS-CoV-2 B.1.525 Spike immobilized at 0.5 ug/mL (100 µL/well)." class="big_thumb" />