RSK1/RSK2 [p Ser221, p Ser227] Antibody [Unconjugated]

RSK1/RSK2 was detected in perfusion fixed frozen sections of mouse brain (Purkinje cells in cerebellum) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S221) and RSK2 (S227) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

RSK1/RSK2 [p Ser221, p Ser227] Antibody [Unconjugated] Summary

• Description: Antibody detects RSK1 [p Ser221] / RSK2 [p Ser227]
• Immunogen: Phosphopeptide containing human RSK1 S221 site
• Modification: p Ser221, p Ser227
• Specificity: Detects human, mouse, and rat RSK1 when phosphorylated at S221, and human and mouse RSK2 when phosphorylated at S227.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Concentration: LYOPH

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RSK1/RSK2 [p Ser221, p Ser227] Antibody [Unconjugated]

• RSK1/RSK2

Background

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RSK1/RSK2 Antibody (AF892)(8)

Publications using AF892

• Jiang FF, Wang RQ, Guo CY et al. Phospho-proteomics identifies a critical role of ATF2 in pseudorabies virus replication Virologica Sinica 6/7/2022 [PMID: 35688418]
• A Bishnu, P Phadte, A Dhadve, A Sakpal, B Rekhi, P Ray Molecular imaging of the kinetics of hyperactivated ERK1/2-mediated autophagy during acquirement of chemoresistance Cell Death & Disease, 2021-02-08;12(2):161. 2021-02-08 [PMID: 33558461] (Western Blot, Human)
• Po-Chien Chou, Swati Rajput, Xiaoyun Zhao, Chadni Patel, Danielle Albaciete, Won Jun Oh et al. mTORC2 Is Involved in the Induction of RSK Phosphorylation by Serum or Nutrient Starvation Cells 2020-06-27 [PMID: 32605013]
• B Nadratowska-Wesolowska, E M Haugsten, M Zakrzewska, P Jakimowicz, Y Zhen, D Pajdzik et al. RSK2 regulates endocytosis of FGF receptor 1 by phosphorylation on serine 789 Oncogene 2014-10-02 [PMID: 24141780]
• Caslavsky J, Klimova Z, Vomastek T ERK and RSK regulate distinct steps of a cellular program that induces transition from multicellular epithelium to single cell phenotype. Cell Signal, 2013-09-03;25(12):2743-51. 2013-09-03 [PMID: 24012955] (Western Blot, Canine)
• Hentschke M, Berneking L, Belmar Campos C Yersinia virulence factor YopM induces sustained RSK activation by interfering with dephosphorylation. PLoS ONE, 2010-10-05;5(10):. 2010-10-05 [PMID: 20957203] (Western Blot, Human, Mouse)
• Meuillet EJ, Zuohe S, Lemos R, Ihle N, Kingston J, Watkins R, Moses SA, Zhang S, Du-Cuny L, Herbst R, Jacoby JJ, Zhou LL, Ahad AM, Mash EA, Kirkpatrick DL, Powis G Molecular pharmacology and antitumor activity of PHT-427, a novel Akt/phosphatidylinositide-dependent protein kinase 1 pleckstrin homology domain inhibitor. Mol. Cancer Ther., 2010-03-02;9(3):706-17. 2010-03-02 [PMID: 20197390] (Western Blot, Human)
• Sindreu CB, Scheiner ZS, Storm DR Ca2+ -stimulated adenylyl cyclases regulate ERK-dependent activation of MSK1 during fear conditioning. Neuron, 2007-01-04;53(1):79-89. 2007-01-04 [PMID: 17196532] (IHC-Fr, Mouse)