Novus Biologicals products are now on bio-techne.com

RGS19 Overexpression Lysate

Images

 
Western Blot: RGS19 Overexpression Lysate (Adult Normal) [NBL1-15325] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RGS19.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RGS19 Overexpression Lysate Summary

Description

RGS19 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC209082

Accession#: NM_005873

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
RGS19

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
24.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RGS19 Overexpression Lysate

  • G alpha interacting protein
  • GAIPG protein signalling regulator 19
  • G-alpha-interacting protein
  • GNAI3IP
  • guanine nucleotide binding protein alpha inhibiting activity polypeptide 3interacting protein
  • regulator of G-protein signaling 19
  • regulator of G-protein signalling 19
  • RGSGAIP

Background

G proteins mediate a number of cellular processes. The protein encoded by this gene belongs to the RGS (regulators of G-protein signaling) family and specifically interacts with G protein, GAI3. This protein is a guanosine triphosphatase-activating protein that functions to down-regulate Galpha i/Galpha q-linked signaling. Alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. Transcript Variant: This variant (1) is the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-91941
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-89489
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-25636
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00008601-M04
Species: Hu, Mu
Applications: ELISA, ICC/IF, S-ELISA, WB
H00005997-M01
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, KD, WB
NB100-1029
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP1-69143
Species: Hu
Applications: WB
NBP1-86020
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-01584
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NLS1017
Species: Bt, Bv, Ca, Eq, Hu, Mu
Applications: IHC, IHC-P
NBP1-91968
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NLS418
Species: Hu
Applications: IHC, IHC-P
NLS2756
Species: Hu, Pm
Applications: ICC, IHC, IHC-P
MAB8458
Species: Hu
Applications: CyTOF-ready, Flow, ICC
NBL1-15325
Species: Hu
Applications: WB

Publications for RGS19 Lysate (NBL1-15325) (0)

There are no publications for RGS19 Lysate (NBL1-15325).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RGS19 Lysate (NBL1-15325) (0)

There are no reviews for RGS19 Lysate (NBL1-15325). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RGS19 Lysate (NBL1-15325) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RGS19 Products

Research Areas for RGS19 Lysate (NBL1-15325)

Find related products by research area.

Blogs on RGS19

There are no specific blogs for RGS19, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RGS19 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol RGS19