Regulatory protein GAL4 Antibody (5C8-5) - Activation Domain - BSA Free

• Reactivity: Ye
• Applications: WB, IP
• Clone: 5C8-5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Regulatory protein GAL4 Antibody (5C8-5) - Activation Domain - BSA Free Summary

• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length Regulatory protein GAL4 expressed in E. Coli and mouse myeloma cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 5-10 ug/ml
• Application Notes: Recognizes Regulatory protein GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions..

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.08% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Regulatory protein GAL4 Antibody (5C8-5) - Activation Domain - BSA Free

• GAL4;GAL81;YPL248C

Background

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.