Recombinant Mouse LRRN4 His-tag Protein, CF Summary
Details of Functionality |
Bioassay data are not available. |
Source |
Mouse myeloma cell line, NS0-derived mouse LRRN4 protein Gln22-Ser676, with a C-terminal 6-His tag |
Accession # |
|
N-terminal Sequence |
Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23 |
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
72 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
110-130 kDa, under reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 500 µg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse LRRN4 His-tag Protein, CF
Background
LRRN4 (Leucine-rich
repeat neuronal protein 4) is a type I transmembrane protein that is a member
of the LRRN family. It is expressed in
lung, heart, ovary, and neuronal tissues (1-3).
Mature mouse LRRN4 is composed of a 657 amino acid (aa) extracellular domain (ECD)
that includes ten LRRs, one LRRCT and a fibronectin type-III like domain, a 21
aa transmembrane segment, and a 36 aa cytoplasmic domain. Within the ECD, mouse LRRN4 shares 66% and
84% aa sequence identity with human and rat LRRN4, respectively.
LRRN4-deficient mice show defects in the memory retention, suggesting this
protein may play an important role in hippocampus-dependent long-lasting memory
(1). In addition, LRRN4 is found in about 8% dorsal root ganglion (DRG) neurons (2).
These neurons are small-sized neurons that function as nociceptors. LRRN4
expression was decreased in the DRG by sciatic axotomy suggesting that LRRN4
might function as a synaptic adhesion molecule to maintain nociceptive circuits
(2). LRRN4 is also expressed in primary mesothelial cells and may be developed
as a maker for detection of mesothelioma antigens (4).
- Bando, T. et al. (2005) Mol. Cell. Biol. 25:4166.
- Bando, T. et al. (2012) Neurosci. Lett. 531:24.
- Bando, T. et al. (2013) Neurosci. Lett. 548:73.
- Kanamori-Katayama, M. (2011) PloS. One. 6(10):e24391.
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