Recombinant Mouse LRRN4 His-tag Protein, CF

2 μg/lane of Recombinant Mouse LRRN4 His-tag (Catalog # 10411-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse LRRN4 His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse LRRN4 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.
• Source: Mouse myeloma cell line, NS0-derived mouse LRRN4 protein
  Gln22-Ser676, with a C-terminal 6-His tag
• Accession #: P59383.2
• N-terminal Sequence: Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-130 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse LRRN4 His-tag Protein, CF

• C20orf75
• chromosome 20 open reading frame 75
• dJ1056H1.1
• FLJ23994
• leucine rich repeat neuronal 4
• leucine-rich repeat neuronal protein 4
• LRRN4
• MGC25027
• Neuronal leucine-rich repeat protein 4
• NLRR4
• NLRR-4

Background

LRRN4 (Leucine-rich repeat neuronal protein 4) is a type I transmembrane protein that is a member of the LRRN family. It is expressed in lung, heart, ovary, and neuronal tissues (1-3). Mature mouse LRRN4 is composed of a 657 amino acid (aa) extracellular domain (ECD) that includes ten LRRs, one LRRCT and a fibronectin type-III like domain, a 21 aa transmembrane segment, and a 36 aa cytoplasmic domain. Within the ECD, mouse LRRN4 shares 66% and 84% aa sequence identity with human and rat LRRN4, respectively. LRRN4-deficient mice show defects in the memory retention, suggesting this protein may play an important role in hippocampus-dependent long-lasting memory (1). In addition, LRRN4 is found in about 8% dorsal root ganglion (DRG) neurons (2). These neurons are small-sized neurons that function as nociceptors. LRRN4 expression was decreased in the DRG by sciatic axotomy suggesting that LRRN4 might function as a synaptic adhesion molecule to maintain nociceptive circuits (2). LRRN4 is also expressed in primary mesothelial cells and may be developed as a maker for detection of mesothelioma antigens (4).

1. Bando, T. et al. (2005) Mol. Cell. Biol. 25:4166.
2. Bando, T. et al. (2012) Neurosci. Lett. 531:24.
3. Bando, T. et al. (2013) Neurosci. Lett. 548:73.
4. Kanamori-Katayama, M. (2011) PloS. One. 6(10):e24391.

Bioinformatics

• Uniprot:
  • P59383.2()