Recombinant Mouse IgG2A Fc Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Clonality: Polyclonal
• Host: Mouse
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse IgG2A Fc Protein, CF Summary

• Description: This product is provided in a carrier free format and does NOT contain BSA.
• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived mouse IgG2A protein Glu98-Lys330, with an N-terminal Met-Asp-Pro
• Accession #: P01863
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Isotype: IgG2a
• Clonality: Polyclonal
• Host: Mouse
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-35 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100 μg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IgG2A Fc Protein, CF

• IgG2A

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Publications for IgG2a (4460-MG)(6)

Publications using 4460-MG

• Hirai, T;Naito, Y;Koyama, S;Nakanishi, Y;Masuhiro, K;Izumi, M;Kuge, T;Naito, M;Mizuno, Y;Yamaguchi, Y;Kang, S;Yaga, M;Futami, Y;Nojima, S;Nishide, M;Morita, T;Kato, Y;Tsuda, T;Takemoto, N;Kinugasa-Katayama, Y;Aoshi, T;Villa, JK;Yamashita, K;Enokida, T;Hoshi, Y;Matsuura, K;Tahara, M;Takamatsu, H;Takeda, Y;Inohara, H;Kumanogoh, A; Sema6D forward signaling impairs T cell activation and proliferation in head and neck cancer JCI insight 2024-02-08 [PMID: 38329122] (Stimulation, Mouse)
• Y He, Y Pei, K Liu, L Liu, Y Tian, H Li, M Cong, T Liu, H Ma, H You, J Jia, D Zhang, P Wang GITR/GITRL reverse signalling modulates the proliferation of hepatic progenitor cells by recruiting ANXA2 to phosphorylate ERK1/2 and Akt Cell Death & Disease, 2022-04-04;13(4):297. 2022-04-04 [PMID: 35379781] (Cell Culture, Rat)
• Ria Aryani Hayuningtyas, Myeonggil Han, Seoyeon Choi, Man Sup Kwak, In Ho Park, Ji-Hyun Lee et al. The collagen structure of C1q induces wound healing by engaging discoidin domain receptor 2 Molecular Medicine 2021-12-01 [PMID: 34602056] (Dot Blot Assay, N/A)
• Hongmin Yun, Michael B. Yee, Kira L. Lathrop, Paul R. Kinchington, Robert L. Hendricks, Anthony J. St. Leger Production of the cytokine VEGF-A by infiltrating CD4+ T and myeloid cells disrupts the corneal nerve landscape and promotes herpes stromal keratitis Immunity 2020-11-04 [PMID: 33207210] (Bioassay, Mouse)
• Y Baglaenko, M Cruz Tleug, E Gracey, N Talaei, KP Manion, NH Chang, DM Ferri, T Mallevaey, JE Wither Invariant NKT Cell Activation Is Potentiated by Homotypic trans-Ly108 Interactions J. Immunol., 2017-04-03;0(0):. 2017-04-03 [PMID: 28373584] (Bioassay, Mouse)
• Matsushita H, Endo S, Kobayashi E, Sakamoto Y, Kobayashi K, Kitaguchi K, Kuroki K, Soderhall A, Maenaka K, Nakamura A, Strittmatter SM, Takai T Differential but competitive binding of Nogo protein and class i major histocompatibility complex (MHCI) to the PIR-B ectodomain provides an inhibition of cells. J. Biol. Chem., 2011-06-02;286(29):25739-47. 2011-06-02 [PMID: 21636572] (Control, Mouse)

Bioinformatics

• Uniprot:
  • P01863()