Recombinant Mouse ELFN1 His-tag Protein, CF

2 μg/lane of Recombinant Mouse ELFN1 His-tag Protein (Catalog # 10644-EF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-84 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse ELFN1 His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived mouse ELFN1 protein
  Gln26-Tyr418, with a C-terminal 6-His tag
• Accession #: Q8C8T7.1
• N-terminal Sequence: Gln26
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 73-84 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ELFN1 His-tag Protein, CF

• ELFN1
• PPP1R28
• USP17L7

Background

Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1 (ELFN1), also known as protein phosphatase 1 regulatory subunit 28 (PPP1R28), is a member of the large family of leucin-rich repeat (LRR) neuronal adhesion protein (1,2).  Mouse ELFN1 consists of an extracellular domain (ECD) containing five LRR domains, one LRR C-terminal (LRRCT) domain and an FN3 domain, a type I transmembrane domain, and a cytoplasmic tail (2). Within the mature ECD, mouse ELFN1 shares 89% and 100% amino acid sequence identity with human and rat ELFN1, respectively. ELFN1 is strongly expressed in globus pallidus and interneurons in cortex and hippocampus in both developing and adult brains and is a selective binding partner of group III glutamate receptors (mGluR) (2, 3). Recent studies showed that ELFN1 physically anchor metabotropic glutamate receptor 6 (mGluR6) and mGluR7 across retinal and hippocampal synapses (3, 4), and can be recruited selectively to all group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) to allosterically modulate these receptors (2). ELFN1 mutant mice display seizures, subtle motor abnormalities, hyperactivity and altered thigmotaxis behavior (4). Given the functions and discrete patterns of many known LRR family proteins it has been proposed that ELFN1 could serve as a neuronal adhesion molecule and play an integral role in synapse formation and differentiation via the coordination of both pre- and postsynaptic machineries, thereby involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (5).

1. Stachniak, T. et al. (2019) J. Neurosci. 39:4461.
2. Dunn, H.A. et al. (2018) Proc. Natl. Acad. Sci. USA 115:5022.
3. Dolan, J. et al. (2007) BMC Genomics 8:320.
4. Dolan, J. and Mitchel, K.J. (2013) PLoS One 8:e8049.
5. Williams, M.E. et al. (2010) Neuron 68:9.

Bioinformatics

• Uniprot:
  • Q8C8T7.1()