Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein, CF

Recombinant Mouse BTNL6/Butyrophilin-like 6 (Catalog # 9638-BT) inhibits IL-2 secretion by mouse T cells in thepresence of anti-CD3 antibody. The ED50 for this effect is 0.15-0.9 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.15-0.9 μg/mL
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse BTNL6 protein
  Lys29-Trp249, with a C-terminal 6-His tag
• Accession #: NP_109672
• N-terminal Sequence: Lys29
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 28-38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BTNL6/Butyrophilin-like 6 Protein, CF

• BTNL6
• Gm6519
• NG13

Background

Butyrophilin-like 6 (BTNL6) is a member of the BTN/MOG Ig-superfamily and functions with BTNL1 as a regulator of immune cell proliferation (1). Mouse BTNL6 is a 539 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a cytoplasmic domain. The ECD features a V-type and a C-type Ig-like domain and one glycosylation site. The Btnl6 gene is found only in mice (2). The ECD of mouse BTNL6 (aa 29-249) shares 47% and 83% sequence identity with the ECD of human BTNL2A2 and rat BTNL9, respectively. BTNL6 expression is coordinated as a heteromeric protein with BTNL1, and the presence of this complex is correlated with expansion of gamma δ T cells, especially those containing V gamma 7Vδ4 TCR (2). Btnl6 shows striking sequence similarity to Skint1; is also largely restricted to an epithelial tissue (the small intestine) replete with T cells (4). Our in-house studies showed BTNL6 co-inhibited anti-CD3 induced IL-2 secretion on CD3⁺ cells.

1. Lebrero-Fernández C. et al. (2016) Front Immunol 7:1.
2. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:559.
3. Lebrero-Fernández C and Bas-Forsberg A. (2016). Sci Rep. 6:31524.
4. Bas, A. et al. (2011) Proc. Natl Acad. Sci. USA 108:4376.

Bioinformatics

• Uniprot:
  • NP_109672()