Recombinant Human TAFA3/FAM19A3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TAFA3/FAM19A3 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human TAFA3/FAM19A3, immobilized at 4 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
• Source: E. coli-derived human TAFA3/FAM19A3 protein
  Ala31-Arg133
• Accession #: Q7Z5A8
• N-terminal Sequence: Ala31
• Protein/Peptide Type: Recombinant Proteins
• Gene: FAM19A3
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 10 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TAFA3/FAM19A3 Protein, CF

• Chemokine-like protein TAFA-3
• FAM19A3
• family with sequence similarity 19 (chemokine (C-C motif)-like), member A3
• MGC138473
• TAFA3
• TAFA-3

Background

TAFA3, also called FAM19A3, is a member of the FAM19/TAFA family of small secreted proteins (1). Human TAFA3 has a molecular weight of approximately 15 kDa. The mature protein is 103 amino acids (aa) in length and shares 93% aa sequence identity with the mouse and rat orthologs (1). Alternative exon splicing causes a reading frame shift at aa 89, creating a 169 aa isoform with an altered C-terminal region (1). TAFA3 is specifically expressed in the brain with the highest levels being detected in parietal cortex and basal ganglia (1). TAFA proteins display chemokine-like signatures and appear to be distantly related to CCL3/ MIP-1 alpha as they share a common CC motif (1). TAFA3 has been suggested to be involved in transmitting the photoperiodically-controlled Melatonin signal from the pars tuberalis (2). TAFA3 mRNA levels in the pars tuberalis vary between night and day and correspond to plasma Melatonin levels (2). Additionally, these day/night expression differences are not observed in mice that lack Melatonin type 1 receptors (2). Interestingly, in other brain regions, such as the hippocampus and the ventral posterior nucleus of the thalamus, TAFA3 expression does not appear to be mediated by Melatonin, suggesting expression of this protein is controlled by region-specific mechanisms (2).

1. Tom Tang, Y. et al. (2004) Genomics 83:727.
2. Fischer, C. et al. (2012) Gen. Comp. Endocrinol. 177:98.

Bioinformatics

• Gene Symbol: FAM19A3
• Uniprot:
  • Q7Z5A8(Human)