Recombinant Human LRRN4 His-tag Protein, CF

2 μg/lane of Recombinant Human LRRN4 His-tag (Catalog # 10227-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-123 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LRRN4 His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human LRRN4 protein
  Asp19-Ser679, with a C-terminal 6-His tag
• Accession #: Q8WUT4
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 106-123 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRRN4 His-tag Protein, CF

• C20orf75
• chromosome 20 open reading frame 75
• dJ1056H1.1
• FLJ23994
• leucine rich repeat neuronal 4
• leucine-rich repeat neuronal protein 4
• LRRN4
• MGC25027
• Neuronal leucine-rich repeat protein 4
• NLRR4
• NLRR-4

Background

LRRN4 (Leucine-rich repeat neuronal protein 4) is a type I transmembrane protein that is a member of the LRRN family. It is expressed in lung, heart, ovary, and neuronal tissues (1-3). Mature human LRRN4 is composed of a 661 amino acid (aa) extracellular domain (ECD) that includes 10 LRRs and a fibronectin type III-like domain, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain. Within the ECD, human LRRN4 shares 66% and 64% aa sequence identity with mouse and rat LRRN4, respectively. LRRN-4-deficient mice show defects in the memory retention, suggesting this protein may be involved in memory retention (4). In addition, LRRN4 is found in about 8% dorsal root ganglion (DEG) neurons (2). These neurons are small-sized neurons that function as nociceptors. LRRN4 expression was decreased in the DRG by sciatic axotomy suggesting that LRRN4 might function as a synaptic adhesion molecule to maintain nociceptive circuits (2). LRRN4 is also expressed in primary mesothelial cells and may be developed as a marker for detection of mesothelioma antigens (5).

1. Bando, T. et al. (2005) Mol. Cell. Biol. 25:4166.
2. Bando, T. et al. (2012) Neurosci. Lett. 531:24.
3. Bando, T. et al. (2013) Neurosci. Lett. 548:73.
4. Bando, T. et al. (1995) Mol. Cellular. Biol. 25:4166.
5. Kanamori-Katayama, M. (2011) PloS. One. 6:e24391.

Bioinformatics

• Uniprot:
  • Q8WUT4()