Recombinant Human FAM20B Protein, CF

• Reactivity: Hu
• Applications: Bioactivity, Enzyme Activity
• Format: Carrier-Free

Recombinant Human FAM20B Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance survival of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.8-4 μg/mL. Measured by its ability to phosphorylate D-xylose. The specific activity is >15 pmol/min/μg, as measured under the described conditions.
• Source: Chinese Hamster Ovary cell line, CHO-derived human FAM20B protein
  Asn27-Leu409, with an N-terminal HA tag
• Accession #: O75063
• N-terminal Sequence: Tyr
• Protein/Peptide Type: Recombinant Proteins
• Gene: FAM20B
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Enzyme Activity
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 44-53 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in 25 mM Tris-HCl, pH7.5.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FAM20B Protein, CF

• EC 2.7.1.-
• FAM20B
• family with sequence similarity 20, member B
• Glycosaminoglycan Xylosylkinase
• gxk1
• KIAA0475
• Protein FAM20B
• XYLK
• Xylose Kinase

Background

FAM20B is a member of the FAM20 protein family that has three members in mammals (FAM20A, FAM20B, FAM20C) (1). Human FAM20B shares 97% amino acid sequence identity with mouse and rat FAM20B. FAM20B localizes to the Golgi apparatus where it phosphorylates the xylose residue in the glycosaminoglycan (GAG)-protein linkage region of proteoglycans, which leads to enhanced GAG biosynthesis (2). Accordingly, chondroitin sulfate and heparan sulfate production is increased when FAM20B is over-expressed and reduced following FAM20B knockdown (2). FAM20B knockout mice display embryonic lethality at day E13.5, suggesting that FAM20B has an important role during development (3). Furthermore, in zebrafish, FAM20B mutations result in reduced cartilage matrix production and skeletal defects (4). The enzymatic activity of recombinant human FAM20B is measured using a phosphatase-coupled method (5).

1. Nalbant, D. et al. (2005) BMC Genomics 6:11.
2. Koike, T. et al. (2009) Biochem. J. 421:157.
3. Vogel, P. et al. (2012) Vet. Pathol. 49:998.
4. Eames, B.F. et al. (2011) PLoS Genet. 7:e1002246.
5. Wu, Z.L. (2011) PLoS One 6:e23172.

Bioinformatics

• Gene Symbol: FAM20B
• Uniprot:
  • O75063()