Recombinant Human ELFN1 His-tag Protein, CF

2 μg/lane of Recombinant Human ELFN1 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 78-121 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ELFN1 His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human ELFN1 protein
  Asp28-Tyr418, with a C-terminal 6-His tag
• Accession #: P0C7U0
• N-terminal Sequence: Asp28
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 78-121 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ELFN1 His-tag Protein, CF

• ELFN1
• PPP1R28
• USP17L7

Background

ELFN1 (Extracellular leucine-rich repeat and fibronectin type-III domain-containing protein 1), also known as Protein phosphatase 1 regulatory subunit 28, is a member of the extracellular Leucine-Rich Repeat superfamily (1). Expressed mainly in the nervous system, ELFN1 is a transmembrane protein that inhibits the activity of protein phosphatase 1 (PP1) complexes (2). Mature human ELFN1 consists of a 391 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment and a 389 aa cytoplasmic tail. The ECD includes one fibronectin type-III domain, six leucine-rich repeats (LRR) and one LRR C-terminal (LRRCT) domain. Human ELFN1 shares 90% aa sequence identity with mouse and rat ELFN1. The cytoplasmic tail contains many tyrosine but no other detectable motifs. ELFN1 is strongly expressed in globus pallidus and interneurons in cortex and hippocampus in both developing and adult brains (1). It is also expressed in endocrine and reproductive tissues (1). Given the functions and discrete patterns of many known LRR family proteins, it has been proposed that ELFN1 could serve as a neuronal adhesion molecule and play an integral role in synapse formation and differentiation via the coordination of both pre- and postsynaptic machineries, thereby involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (3). Recent studies showed that ELFN1 physically anchor metabotropic glutamate receptor 6 (mGluR6) and mGluR7 across retinal and hippocampal synapses (3-4), and can be recruited selectively to all group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) to allosterically modulate these receptors (5).

1. Dolan J. et al. (2007) BMC Genomics 8:320.
2. Hendrickx, A. et al. (2009) Chem. Biol. 16:365.
3. Williams, M.E. et al. (2010) Neuron 68:9.
4. De Wit, J. and Ghosh A. (2016) Nat Rev Neurosci 17:22.
5. Dunn H. A. et al. (2018) PNAS 115:5022.

Bioinformatics

• Uniprot:
  • P0C7U0()