RecA-1 Antibody (HIS52) [Alexa Fluor™ Plus 405]

• Reactivity: Rt
• Applications: IHC, ICC/IF
• Clone: HIS52
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 405

RecA-1 Antibody (HIS52) [Alexa Fluor™ Plus 405] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Stromal cells from rat lymph node
• Specificity: Recognizes RECA-1, a cell surface antigen which is expressed by all rat endothelial cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB100-64647. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Does not react with:Goat, Chicken, Guinea Pig, Sheep, Mouse, Rabbit, Pig

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RecA-1 Antibody (HIS52) [Alexa Fluor™ Plus 405]

• RecA protein
• RecA1
• RecA2
• recaLP
• Recombinase A

Background

RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognizes a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell specific antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.