Raw 264.7 Whole Cell Lysate

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Western Blot: Raw 264.7 Whole Cell Lysate [NBP1-30158] - Analysis of ABCA1, using NB400-105. 25 ug of whole cell lysate of RAW 264.7 cells either treated with T0901317 (NBP1-30158 lane 1) or untreated (NBP1-30157 lane ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Concentration
1.0 mg/ml

Order Details

Raw 264.7 Whole Cell Lysate Summary

Localization
Macrophage

Applications/Dilutions

Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.
Positive Control
ABCA1 Antibody (NB400-105)
ABCA1 Antibody (NB400-164)
ABCA1 Antibody (NB400-165)
ABCA1 Antibody (NB100-1663)
ABCA1 Antibody (NB100-2068)

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added.
Concentration
1.0 mg/ml

Lysate Details for Raw 264.7

Type
Cell
Tissue
Blood Lymph
Protein State
Denatured
Subcellular Fraction
Whole

Notes

NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Large protein samples should be heated to 65C for 5-10 minutes prior to loading.

Background

T0901317 is an LXR agonist which acts to upregulate ABCA1 gene expression and promotes reverse cholesterol transport from macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Additional Raw 264.7 Products

Raw 264.7 NBP1-30158

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ABCA1 Antibody
NB400-105

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