Raw 264.7 Whole Cell Lysate


Western Blot: Raw 264.7 Whole Cell Lysate [NBP1-30158] - Analysis of ABCA1, using NB400-105. 25 ug of whole cell lysate of RAW 264.7 cells either treated with T0901317 (NBP1-30158 lane 1) or untreated (NBP1-30157 lane ...read more

Product Details

Applications WB
1.0 mg/ml

Order Details

Raw 264.7 Whole Cell Lysate Summary


Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added.
1.0 mg/ml


  • Western Blot
Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Lysate Details for Raw 264.7

Blood Lymph
Protein State
Subcellular Fraction


NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Large protein samples should be heated to 65C for 5-10 minutes prior to loading.


T0901317 is an LXR agonist which acts to upregulate ABCA1 gene expression and promotes reverse cholesterol transport from macrophages.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Positive Control Lysate(s)

Additional Raw 264.7 Whole Cell Lysate Products

Raw 264.7 NBP1-30158

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