Rae-1 epsilon Antibody (205001)

BaF3 mouse pro-B cell line transfected with (A) mouse Rae-1 epsilon or (B) mouse Rae-1 alpha and eGFP was stained with Rat Anti-Mouse Rae‑1 epsilon Monoclonal Antibody (Catalog # MAB1135) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

• Reactivity: Mu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 205001
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Rae-1 epsilon Antibody (205001) Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with mouse Rae-1 epsilon
• Specificity: Detects mouse Rae‑1 epsilon in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Rae-1 alpha , beta , gamma , or δ is observed. By flow cytometry, no cross-reactivity with mouse Rae-1 alpha or mouse Rae-1 gamma .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Raet1e
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rae-1 epsilon Antibody (205001)

• Rae1 epsilon
• Rae-1 epsilon

Background

Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha , beta , gamma , and δ. Amino acid sequence identity within this family ranges from 88‑95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig‑like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI‑linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10‑14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑7).

1. Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
3. Cerwenka, A. et al. (2000) Immunity 12:721.
4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
5. Diefenbach, A. et al. (2001) Nature 413:165.
6. Champsaur, M. et al. (2010) J. Immunol. 185:157.
7. Markiewicz M. et al. (2012) Immunity 36:132.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rae-1 epsilon Antibody (MAB1135)(5)

Publications using MAB1135

• I Kavazovi?, M Lenarti?, V Jelen?i?, S Jurkovi?, NA Lemmermann, S Jonji?, B Poli?, FM Wensveen NKG2D stimulation of CD8+ T cells during priming promotes their capacity to produce cytokines in response to viral infection in mice Eur. J. Immunol., 2017-05-02;0(0):. 2017-05-02 [PMID: 28378389] (Flow Cytometry, Mouse)
• Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice. Immunology, 2014-03-01;141(3):401-15. 2014-03-01 [PMID: 24708417] (Flow Cytometry, Mouse)
• Jung , Heiyoun, Hsiung , Benjamin, Pestal , Kathleen, Procyk , Emily, Raulet , David H RAE-1 ligands for the NKG2D receptor are regulated by E2F transcription factors, which control cell cycle entry. J Exp Med, 2012-11-19;209(13):2409-22. 2012-11-19 [PMID: 23166357] (IHC-Fr, Mouse)
• Markiewicz MA, Wise EL, Buchwald ZS, Pinto AK, Zafirova B, Polic B, Shaw AS RAE1epsilon ligand expressed on pancreatic islets recruits NKG2D receptor-expressing cytotoxic T cells independent of T cell receptor recognition. Immunity, 2012-01-12;36(1):132-41. 2012-01-12 [PMID: 22244846] (IHC-Fr, Mouse)
• Unni AM, Bondar T, Medzhitov R Intrinsic sensor of oncogenic transformation induces a signal for innate immunosurveillance. Proc. Natl. Acad. Sci. U.S.A., 2008-01-25;105(5):1686-91. 2008-01-25 [PMID: 18223157] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Raet1e