PVRIG Antibody (2334B) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human PVRIG or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human PVRIG Monoclonal Antibody (Catalog # MAB93652) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

In a functional ELISA, Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) binds to immobilized Recombinant Human PVRIG Fc Chimera (Catalog # 9365-PV) coated at 1 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PVRIG Monoclonal Antibody (Catalog # MAB93652). At 2.5 µg/mL, this antibody will block >90% of the binding.

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 2334B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PVRIG Antibody (2334B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human PVRIG
  Thr41-Leu172
  Accession # Q6DK17
• Specificity: Detects human PVRIG in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PVRIG Antibody (2334B) [Unconjugated]

• C7orf15
• C7orf15MGC138295
• CD112R
• MGC104322
• MGC138297
• MGC2463
• poliovirus receptor related immunoglobulin domain containing
• Poliovirus receptor-related immunoglobulin domain-containing protein
• PVRIG
• transmembrane protein PVRIG

Background

Human PVRIG (Poliovirus Receptor Related Immunoglobulin Domain-containing Protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1).

1. Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q6DK17()