PSTAIR Antibody (PSTAIR)

Western Blot: PSTAIR Antibody (PSTAIR) [NB120-10345] - Cell line lysates were separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-PSTAIR Clone:PSTAIR. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. SK-N-SH 4. SH-SY5Y 5. U87 6. COS7 7. PC12 8. Neuro-2a 9. Arabidopsis

• Reactivity: Hu, Mu, Av, Pl, Pm, Xp
• Applications: WB, ELISA, IP
• Clone: PSTAIR
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.

PSTAIR Antibody (PSTAIR) Summary

• Description: Novus Biologicals Mouse PSTAIR Antibody (PSTAIR) (NB120-10345) is a monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide: EGVPSTAIREISLLKE conjugated to BSA.
• Epitope: Recognizes an epitope present in the PSTAIR sequence 1 of p34cdc2 (cdk1) and of other cyclin-dependent kinases containing the PSTAIR motif (cdk2 and cdk3).
• Specificity: PSTAIR (PSTAIR)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. It cannot precipitate p34cdc2; when it is complexed with cyclin B.WB: Use at a dilution of 1/4000 (determined by indirect blotting using COS-7 cell extract). The antibody recognises 31-34kD proteins (1-4) bands.Monoclonal Anti-PSTAIR may be used for the localization of cyclin-dependent kinases, containing the PSTAIR motif.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with human, monkey, mouse, Japanese quail, fish (goldfish, carp, eel, starfish, and amago salmon), Xenopus, cliliates, and plants (lily and onion). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Raw ascites
• Preservative: 0.1% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Unpurified

Alternate Names for PSTAIR Antibody (PSTAIR)

• EGVPSTAIREISLLKE sequence
• EGVPSTAIREISLLKE
• PSTAIR sequence

Background

The cell cycle is controlled in part by cyclin-dependent kinases (CDKs) which are themselves controlled by modifications such as phosphorylation of CDKs and formation of complex(es) with other proteins, including the cyclins. CDKs are key regulators of cell cycle progression. CDKs are closely related in size (35-40 kD) and sequence (>40% identity) and associate with and are activated by a cyclin which acts as a regulatory subunit. In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in the complex formation with cyclins. The availability of antibodies reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for PSTAIR Antibody (NB120-10345). (Showing 1 - 1 of 1 FAQ).

1. Does this antibody also recognize Cdc28-PSTAIR from budding yeast (S. cerevisiae) lysate?
   • NB120-10345 is predicted to have cross reactivity with a wide range of species based on the sequence homology, although we haven't tested it specifically in yeast, but I did a blast search and it has 100% homology with S. cerevisiae cdk1 so most likely it should work in yeast as well.