Protocadherin alpha 1 Antibody

Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protocadherin a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Protocadherin a1 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Protocadherin alpha 1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 10 µg/mL Sheep Anti-Human Protocadherin alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counter­stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Protocadherin alpha 1 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Human Protocadherin alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5064) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counter­stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Protocadherin alpha 1 Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Protocadherin alpha 1
  Gln30-Pro689
  Accession # Q9Y5I3
• Specificity: Detects human Protocadherin alpha 1 (PCDH alpha 1) in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PCDHA1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protocadherin alpha 1 Antibody

• KIAA0345-like 13
• PCDHA1
• PCDH-ALPHA1
• PCDH-alpha-1
• Protocadherin alpha 1
• protocadherin alpha-1

Background

Protocadherin alpha 1 (PCDH alpha 1) is a group A member of the Protocadherin family of molecules. It is expressed in synaptic membranes of neurons, forms obligate Ca⁺⁺ independent complexes with various PCDH  gamma family members and demonstrates heterophilic binding activity towards beta 1 integrins. Human PCDH alpha 1 is 921 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 668 aa extracellular domain (ECD) plus a 232 aa cytoplasmic region. Both regions contribute to PCDH-gamma binding. There are six cadherin domains in the ECD. ADAM10 cleavage generates an 80‑90 kDa soluble fragment and a soluble splice form exists that is missing aa 535‑798. Over aa 30‑689, human PCDH alpha 1 shares 83% aa identity with mouse PCDH alpha 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCDHA1
• Uniprot:
  • Q9Y5I3()