Progesterone ELISA Kit (Colorimetric)

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ELISA: Progesterone ELISA Kit (Colorimetric) [NBP2-61290] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Applications ELISA
Suitable Sample Type
Culture supernatants, serum, and saliva from any species
Standard Curve Range
15.62 - 500 pg/ml
Sensitivity
8.57 pg/ml

Order Details

Progesterone ELISA Kit (Colorimetric) Summary

Standard Curve Range
15.62 - 500 pg/ml
Sensitivity
8.57 pg/ml
Assay Type
Competitive ELISA
Inter-Assay
Mean 5.9%
Intra-Assay
Mean 5.9%
Spike Recovery
Tissue Culture Media - 105.0%
Human Saliva - 98.1%
Human Serum - 106.4%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Antibody
  2. Assay buffer
  3. Conjugate
  4. GxM IgG Microtiter plate
  5. pNpp Substrate
  6. Standard
  7. Steroid displacement reagent
  8. Stop solution
  9. Wash buffer concentrate

Alternate Names for Progesterone ELISA Kit (Colorimetric)

  • 4-Pregnen-3,20-dione
  • Progesterone

Background

Progesterone is the major female sex hormone, and is responsible for reproductive-related activities such as breast glandular development, the endometrial aspects of the menstrual cycle, and the establishment and maintenance of pregnancy. In addition, progesterone also directs pregnancy support physiology including changes in carbohydrate, protein and lipid metabolism, thermoregulation, sodium reabsorption in renal tubules and the reduction of alveolar and atrial carbon dioxide partial pressures (pCO2). Progesterone is secreted in large amounts by the corpus luteum and by the extracellular conversion of cholesterol, cholestryl esters, adrenal steroids, prenenolone and pregnenolone sulfate. Small quantities are also secreted directly from the adrenal glands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Progesterone ELISA Kit (NBP2-61290). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Autophagy, also known as macroautophagy, supplies alternative fuel for cells that are under environmental stress conditions (including starvation, growth factor deprivation, and hypoxia). This highly regulated and catabolic cell process recycles a...  Read full blog post.

PMP22 Antibodies Assist in PNS Myelin Repair Research
Novus Biologicals recently added a new  PMP22 antibody, conjugated to Biotin, to our antibody database. PMP22 (Peripheral Myelin Protein 22) is important to the structure of the myelin sheath in peripheral nerves, and is encoded by the PMP22 gene.Th...  Read full blog post.

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