Mouse Progesterone ELISA Kit (Colorimetric)

Mouse Progesterone ELISA Kit (Colorimetric) [NBP2-60125] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum and plasma
• Standard Curve Range: 0.3 ng/mL - 10 ng/mL
• Sensitivity: 0.2 ng/mL

Mouse Progesterone ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse PROG. No significant cross-reactivity or interference between mouse PROG and analogues was observed.
• Standard Curve Range: 0.3 ng/mL - 10 ng/mL
• Sensitivity: 0.2 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Progesterone ELISA Kit (Colorimetric)

• 4-Pregnen-3,20-dione
• Progesterone

Background

This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PROG and Horseradish Peroxidase (HRP) conjugated PROG. The competitive inhibition reaction is launched between with HRP labeled PROG and unlabeled PROG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PROG in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Progesterone ELISA Kit (NBP2-60125)(3)

Publications using NBP2-60125

• Miriam Ferrer, Nicholas Mourikis, Emma E. Davidson, Sam O. Kleeman, Marta Zaccaria, Jill Habel, Rachel Rubino, Qing Gao, Thomas R. Flint, Lisa Young, Claire M. Connell, Michael J. Lukey, Marcus D. Goncalves, Eileen P. White, Ashok R. Venkitaraman, Tobias Janowitz Ketogenic diet promotes tumor ferroptosis but induces relative corticosterone deficiency that accelerates cachexia Cell metabolism 2024-04-23 [PMID: 37311455]
• Topilko T, Diaz SL, Pacheco CM et al. Edinger-Westphal peptidergic neurons enable maternal preparatory nesting Neuron 2022-02-01 [PMID: 35123655] (ELISA)
• Zhou Y, Zhang A, Gong M et al. Maternal testosterone excess contributes to reproductive system dysfunction of female offspring mice Endocrinology 2019-11-04 [PMID: 31680156] (ELISA, Mouse)

FAQs for Progesterone ELISA Kit (NBP2-60125). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.