Equine Progesterone ELISA Kit (Colorimetric)

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ELISA: Equine Progesterone ELISA Kit (Colorimetric) [NBP2-60119] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity EqSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
0.25 ng/ml-100 ng/ml
Sensitivity
0.25 ng/ml

Order Details

Equine Progesterone ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of horse PROG. No significant cross-reactivity or interference between horse PROG and analogues was observed.
Standard Curve Range
0.25 ng/ml-100 ng/ml
Sensitivity
0.25 ng/ml
Assay Type
Competitive ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 15%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. HRP-conjugate
  2. Instruction manual
  3. Antibody
  4. Assay plate
  5. Adhesive Strip (For 96 wells)
  6. Wash Buffer (20 x concentrate)
  7. Substrate B
  8. Substrate A
  9. Standard
  10. Stop Solution

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Equine Progesterone ELISA Kit (Colorimetric)

  • 4-Pregnen-3,20-dione
  • Progesterone

Background

This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PROG and Horseradish Peroxidase (HRP) conjugated PROG. The competitive inhibition reaction is launched between with HRP labeled PROG and unlabeled PROG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PROG in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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